DNA Synthesis

1st BASE Custom Oligos (FBCO) produces a comprehensive range of oligonucleotides to meet your research needs.

Our PCR-grade oligos are desalted and can be used in most research applications such as PCR, RT-PCR, and sequencing.

For more demanding applications such as mutagenesis, we provide purification options to ensure you achieve greater experimental success rates. A specialized FBCO team handles the full spectrum of your oligo modification needs, from commonly used fluorophores to attachment linkers, as well as rarer modifications. With “Quality, Speed, and Convenience” as our motto, you can expect only the best from us.

Features of FBCO DNA Oligos

• Each oligo is deprotected and desalted to give more consistent results
• Standard delivery format – resuspended to 100 µM in nuclease-freeH20
• Optional delivery formats – lyophilized OR resuspeneded to 100 µM in 1X TE Buffer (pH 8.0)
• Comprehensive datasheet provided
• Bar-coded labels on oligo tubes for easy automation
• 1X TE Buffer provided with lyophilized oligos (for local customers)
• No set-up charge
• No additional charge for equimolar degeneracies
• Primer Design Services available (additional charges may apply)
• Prompt delivery (inclusive of Saturday deliveries for local customers, subject to prior arrangements)
• Expedite Service available, please enquire

FBCO’s 5-step QC process

Our 5-Step Quality Control checks are put in place to monitor production processes at every stage so that your research is not compromised in any way. All of our oligos pass through stringent testing using MALDI- ToF mass spectrometry so that you are assured of high quality products.

• Trityl monitoring at 3 stages during oligo synthesis process


• All completed oligos undergo Mass Spectrometry Quality Analysis, where they are benchmarked against our acceptance criteria of ± 0.2% and ± 0.5% of the calculated molecular mass for below 30mer and above 30mer base lengths, respectively


• For oligos not suitable for MS analysis, HPLC or PAGE analysis is used


• Oligos are functionally tested by weekly in-house PCR tests


• QC Recall Programme - Our stringent Pre-delivery QC ensures that only the highest quality oligos are delivered. For post-delivery QC, should we suspect that quality is compromised, customers will be alerted immediately and a priority replacement will be made.