qPCR Assays and Probes

Quantitative real-time PCR (qPCR) is now used routinely as an important analytical tool in the life sciences. qPCR allows for gene expression profiling and real-time quantification, providing data for the following applications:

Gene expression profiling

• Mutation detection
   
• Allelic discrimination
   
• Single Nucleotide Polymorphisms (SNPs)
   
• Pathogen detection
   

Quantification

• Melting curve profiles
   
• Single or multiplex assays
   
• Universal cycling conditions
   
• High-throughput screening assays
   

We offer PrimeTime® qPCR Probes and Assays containing high quality dual-labeled hydrolysis probes used for routine qPCR. PrimeTime qPCR Probes are non-extendable oligonucleotides labeled with a 5’ fluorescent reporter and a 3’ quencher dye. These are available as DNA or DNA mixed with LNA bases.The Probes are licensed for use in the 5’ nuclease real-time PCR assay (with companion dye and quencher licenses). PrimeTime qPCR Probes are available with a variety of reporter/quencher combinations and synthesis scales.

PrimeTime qPCR Assays

• Receive primers and probe premixed and delivered in a single tube
   
• Use the easy ordering tools for guaranteed performance
   
• Select from 5 quencher combinations and 3 reactions scales
  Choose ZEN™ Double-Quenched Probes in your assay for superior performance compared to traditional dual-labeled probes 

Order Assays online now

PrimeTime qPCR Probes

• Get dual-labeled probes with a wide variety of dyes and quenchers
   
• Order Express qPCR Probes for shipment in a single work day
   
• Try Ultra-small-scale Mini qPCR Probes to test a new probe or to screen the expression levels of many genes
   
• Choose ZEN Double-Quenched Probes for superior performance compared to traditional dual-labeled probes 
   

Order Probes online now

Visit http://eu.idtdna.com/catalog/primetime/primetime.aspx for more product details and technical resources.