RNAi Synthesis

RNA Interference (RNAi) is a mechanism by which short strands of double-stranded RNA inhibit the expression of genes with complementary nucleotide sequences. It is a naturally occurring cellular mechanism in many eukaryotes and can be used by researchers to specifically target and silence genes of interest.

The term RNAi was coined after Craig Mello and Andrew Fire reported in 1998 that injection of double-stranded RNA into C. elegans cells was able to silence a target gene. Mello and Fire were awarded the Nobel Prize in Physiology or Medicine in 2006 for their groundbreaking discovery.

The past decade has seen increasing use of short interfering RNA (siRNA) and RNAi as a tool to study functional genomics, developmental biology, gene expression and in vivo and in vitro transfection efficiency studies.

At 1st BASE Custom Oligos (FBCO), we offer the following IDT products for gene silencing and RNAi experiments:

• Custom Dicer-substrate siRNAs (DsiRNAs)
• Pre-designed DsiRNAs
• TriFecta RNAi Kit
• RNAi Duplex Oligos
• Antisense Oligonucleotides